Coding

Part:BBa_K3715117:Design

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-26)


PETase_MT12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to improve the stability of the enzyme, we engineered PETase through multiple site mutations.


Source

We make multiple site mutations on the basis of WT（PETase）which is from the PET-consuming microbeIdeonella sakaiensis.

References