Composite

Part:BBa_K3715054:Design

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-26)


T7 promoter+RBS+Linker a+His+PETase_MT16+Linker b+T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 954
    Illegal XhoI site found at 968
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NCBI



Source

This part is codon optimized in E. coli DH5α.


References