Composite

Part:BBa_K3715023:Design

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-27)


T7 promoter+RBS+ His+Linker g+MBP+Linker c+PETase_MT9+Linker d+T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 70
    Illegal PstI site found at 2080
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 70
    Illegal PstI site found at 2080
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 441
    Illegal XhoI site found at 2087
    Illegal XhoI site found at 2101
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 70
    Illegal PstI site found at 2080
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 70
    Illegal PstI site found at 2080
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 139


Design Notes

This part is codon optimized in E. coli BL21.


Source

NCBI


References