Composite

Part:BBa_K3711067

Designed by: Jiacheng Shi   Group: iGEM21_HUST-China   (2021-10-03)


Pynr071c-ROX1-AOX1 Terminator


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2438
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1357
    Illegal SapI site found at 1616


Description

A kind of yeast protein, which could inhibit promoter Panb1 and then silence following genes of it. This is a composite part for intracellular expression of ROX1. Pynr071c is the promoter of Aldose 1-isomerase superfamily protein YNR071C. In the presence of xylose, ROX1 is expressed and participates in the inhibit promoter Panb1 and then silence following genes of it.

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