Plasmid_Backbone
Part:BBa_K371051
Designed by: Ruijun Zhu Group: iGEM10_USTC (2010-10-27)
pSB1C5 (1bp mutation of pSB1C3 to remove SacI)
This part is a plasmid bone, which has only a single site mutation compared with pSB1C3. The SacI site is mutated to allow this plasmid compatible to BBF RFC 53 standard.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2051
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2057 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2051
Illegal XhoI site found at 1034
Illegal XhoI site found at 1927 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2051
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2051
Plasmid lacks a suffix.
Illegal XbaI site found at 2066
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
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Categories
Parameters
None |