Plasmid_Backbone

Part:BBa_K371051

Designed by: Ruijun Zhu   Group: iGEM10_USTC   (2010-10-27)

pSB1C5 (1bp mutation of pSB1C3 to remove SacI)

This part is a plasmid bone, which has only a single site mutation compared with pSB1C3. The SacI site is mutated to allow this plasmid compatible to BBF RFC 53 standard.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2051
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2051
    Illegal XhoI site found at 1034
    Illegal XhoI site found at 1927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2051
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2051
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2066
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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Categories
Parameters
None