Protein_Domain

Part:BBa_K371024:Design

Designed by: Yang Zhang   Group: iGEM10_USTC   (2010-10-27)

10*GS linker(for RFC 53 protein fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Different codons of Gly and Ser was choosed in order to ignore the mismatch between forward sequence and reverse sequence when they are annealing.Here is the oligonucleotides used to generate 10*GS.

forward sequence:

5'-GGTGGTAGCGGCAGCGGTAGCGGTAGCGGCAGC-3'

reverse sequence:

5'-CATGCTGCCGCTACCGCTACCGCTGCCGCTACC-3'

Then the product can directly be used to ligate with protein domain to make fusion protein according to BBF RFC 53.

Source

This part is not long, so it can be easily get by direct annealing two oligonucleotides.

References