Composite

Part:BBa_K371019:Design

Designed by: Yang Zhang, Ruijun Zhu   Group: iGEM10_USTC   (2010-10-11)

pdu(Propanediol utilization)BMC shell gene under Plac control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1894
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3236
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To get this BioBrick, we split this part into five part: promotor, pduAB, pduJK, pduN, pduTUV.Promotor BBa_R0010 comes from parts.igem and was chosen to give a mediate transcription efficiency after induced by IPTG. Pdu AB, pduJK, pduN and pduTUV come from Citrobacter Freundii genome by Polymerase Chain Reaction(PCR) and the inappropri ate restriction sites were moved before continuing next step assembling. Here is the assembling map of our project.

PduABJKNTUV RFC 10 assembly.png

Source

Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkm

References