Translational_Unit

Part:BBa_K371005

Designed by: Yang Zhang   Group: iGEM10_USTC   (2010-08-27)


pduTUV(Propanediol utilization gene T+U+V)[RBS+pduT+RBS+pduU+RBS+pduV]

Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes, which belongs to a kind of BMC(bacterial microcompartment).Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduTUV consist of pduT, pduU and pduV wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduTUV, together with other struture parts of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 815
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apduTUV(Propanediol utilization gene T+U+V)[RBS+pduT+RBS+pduU+RBS+pduV]
originCitrobacter Freundii