Composite
Urea Senso

Part:BBa_K3710004:Design

Designed by: Sandra Lizeth Martinez Diaz, Erik Hanko and Katherine Baker   Group: iGEM21_Manchester   (2021-10-01)


Urea Biosensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1982
    Illegal AgeI site found at 2094
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pUreD is a bidirectional promoter driving transcription of the UreR and UreD protein subunits of the urease operon of P. mirabilis. pUreD contains two ribosome binding sites and a UreR binding site about upstream of the forward transcriptional start site.


Source

Urease Operon of Proteus mirabilis. The sequences were drawn from the gene Bank NCBI. The pUreD sequence corresponds to the intergenic region between the UreR and UreD subunits of the Urease Operon.

References