DNA

Part:BBa_K3696015:Design

Designed by: Yuanhong Pan   Group: iGEM20_OSA   (2020-10-26)


A type of DNA oligonucleotide that can cleave towards its substrate only after binding to Pb2+.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The actual sequence of BBa_K3696015 is ATAGAGAATTGTAACTGAAGTAGCGCCGCCGTATAGTGAGAAAACTCACTAT rA GGAAGTTACAATTCTCTAT. "r" means the base after it is a RNA base. Furthermore, in the sequence design of BBa_K3696015, the fluorescence group was not added to the sequence due to difficulties in synthesis.


Source

The sequence of BBa_K3696015 is artificially designed. We adopted the sequence from the article "Nucleic acid-cleaving catalytic DNA for sensing and therapeutics" and synthesized the part accordingly.

References