Part:BBa_K3696002

The cleavable substrate of 8-17 DNAzyme.

BBa_K3696000, BBa_K3696002--BBa_K3696003 are used in HTDC testings, the protocol is shown below:

HTDC protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

HTDC Pb2+ testing

From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.


BBa_K3696001, BBa_K3696002 are used in 8-17 DNAzyme testings, the protocol is shown below:

8-17 DNAzyme protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

8-17 DNAzyme(two parts) Pb2+ testing

From the detected fluorescence, we determine that: 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]