DNA

Part:BBa_K3696000

Designed by: Chaohan Ji   Group: iGEM20_OSA   (2020-10-25)


A type of DNA oligonucleotide that can cleave towards its substrate after binding to target and Pb2+

BBa_K3696000, BBa_K3696002--BBa_K3696003 are used in HTDC testings, the protocol is shown below:

HTDC protocol


The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

HTDC Pb2+ testing

From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None