Composite

Part:BBa_K3693011:Design

Designed by: Jie Gao   Group: iGEM20_BJ101HS   (2020-10-22)


Purification of LALBA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add his-tag at both N-terminal and C-terminal for affinity purification


Source

The his-tag is we designed and added artificially, the gene it self is from Homo sapiens but we did codon optimization.

References