Composite

Part:BBa_K3693004:Design

Designed by: Jie Gao   Group: iGEM20_BJ101HS   (2020-10-19)


For secreting the κ-casein outside yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add a his tag between secretion signal and gene for purification.


Source

The alpha-secretion signal is from pichia pastoris. His-tag is we designed and added artificially. The original sequence of CSN3 is from Homo sapiens but we did codon optimization.

References