Coding

Part:BBa_K3692004

Designed by: Juli Mukhadze   Group: iGEM20_Estonia_TUIT   (2020-10-07)


SAG1

Usage and Biology

Sag1 is one of the cell wall anchored proteins that contain the GPI-anchoring domain.

The presence of Sag1 inside the cell wall was indicated by the fusion of the Sag1-anchoring domain with eGFP (Inokuma et al., 2020).

It has been shown that the anchoring domain from different GPI cell wall proteins exhibit different efficiencies for the cell-surface display of target enzymes (Andreu & del Olmo, 2018)

It was observed by our team that induced expression of bacterial glucanases has the potential to cause lysis in Sacharomyces cerevisiae, (Team:Tartu TUIT - 2019.Igem.Org, n.d.). To boost the efficiency of autolysis, the localization of glucanases can be improved by fusing them with GPI-CWPs (Inokuma et al., 2020).

References

  • Inokuma, K., Kurono, H., den Haan, R., van Zyl, W. H., Hasunuma, T., & Kondo, A. (2020). Novel strategy for anchorage position control of GPI-attached proteins in the yeast cell wall using different GPI-anchoring domains. Metabolic Engineering, 57, 110–117. https://doi.org/10.1016/j.ymben.2019.11.004
  • Andreu, C., & del Olmo, M. (2018). Yeast arming systems: pros and cons of different protein anchors and other elements required for display. In Applied Microbiology and Biotechnology (Vol. 102, Issue 6, pp. 2543–2561). Springer Verlag. https://doi.org/10.1007/s00253-018-8827-6
  • Team:Tartu TUIT - 2019.igem.org. (n.d.). Retrieved October 16, 2020, from https://2019.igem.org/Team:Tartu_TUIT

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 73
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 493


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Parameters
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