Coding

Part:BBa_K3692001

Designed by: Juli Mukhadze   Group: iGEM20_Estonia_TUIT   (2020-10-07)


PAH1

Usage and Biology

PAH1 is a Saccharomyces cerevisiae gene which encodes phosphatidic acid phosphohydrolase 1, or Pap1. Pap1 plays a role in the synthesis of triacylglycerols (TAGs) by converting phosphatidic acid, PA into diacylglycerols, DAG.

The recruitment of Pap1 to the ER membrane depends mainly on PA levels in the ER. However, it was shown that additional factors can force Pah1 recruitment (Karanasios et al., 2010).

The transmembrane protein phosphatase complex Nem1p-Spo7p is the main regulator of Pah1 activity. It consists of two subunits Nem1, the catalytic subunit of the complex, and Spo7 whose function is less clear.

Pah1 dephosphorylation enables the binding to ER membrane via a short amino-terminal amphipathic helix to the membrane. This interaction is important for both phospholipid and TAG biosynthesis (Santos-Rosa et al., 2005).

The overexpression of PAH1 in S. cerevisiae was found to increase TAG levels by 3.9-fold (Hung et al., 2016).

References

  • Karanasios, E., Han, G. S., Xu, Z., Carman, G. M., & Siniossoglou, S. (2010). A phosphorylation-regulated amphipathic helix controls the membrane translocation and function of the yeast phosphatidate phosphatase. Proceedings of the National Academy of Sciences of the United States of America, 107(41), 17539–17544. https://doi.org/10.1073/pnas.1007974107
  • Santos-Rosa, H., Leung, J., Grimsey, N., Peak-Chew, S., & Siniossoglou, S. (2005). The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO Journal, 24(11), 1931–1941. https://doi.org/10.1038/sj.emboj.7600672
  • Hung, C. H., Kanehara, K., & Nakamura, Y. (2016). In vivo reconstitution of algal triacylglycerol production in Saccharomyces cerevisiae. Frontiers in Microbiology, 7(FEB). https://doi.org/10.3389/fmicb.2016.00070

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 733
    Illegal EcoRI site found at 909
    Illegal EcoRI site found at 2436
    Illegal EcoRI site found at 2545
    Illegal EcoRI site found at 2560
    Illegal EcoRI site found at 2575
    Illegal SpeI site found at 2332
    Illegal PstI site found at 2321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 733
    Illegal EcoRI site found at 909
    Illegal EcoRI site found at 2436
    Illegal EcoRI site found at 2545
    Illegal EcoRI site found at 2560
    Illegal EcoRI site found at 2575
    Illegal SpeI site found at 2332
    Illegal PstI site found at 2321
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 733
    Illegal EcoRI site found at 909
    Illegal EcoRI site found at 2436
    Illegal EcoRI site found at 2545
    Illegal EcoRI site found at 2560
    Illegal EcoRI site found at 2575
    Illegal BglII site found at 1292
    Illegal BamHI site found at 2161
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 733
    Illegal EcoRI site found at 909
    Illegal EcoRI site found at 2436
    Illegal EcoRI site found at 2545
    Illegal EcoRI site found at 2560
    Illegal EcoRI site found at 2575
    Illegal SpeI site found at 2332
    Illegal PstI site found at 2321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 733
    Illegal EcoRI site found at 909
    Illegal EcoRI site found at 2436
    Illegal EcoRI site found at 2545
    Illegal EcoRI site found at 2560
    Illegal EcoRI site found at 2575
    Illegal SpeI site found at 2332
    Illegal PstI site found at 2321
    Illegal NgoMIV site found at 1576
    Illegal NgoMIV site found at 2374
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None