Part:BBa_K3683002:Design
S(1-1254aa) DNA D614G
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3094
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1435
Illegal NgoMIV site found at 1951
Illegal NgoMIV site found at 2014
Illegal NgoMIV site found at 2509
Illegal NgoMIV site found at 2653
Illegal NgoMIV site found at 2686
Illegal NgoMIV site found at 2963 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 647
Illegal BsaI.rc site found at 898
Illegal BsaI.rc site found at 2558
Illegal SapI.rc site found at 915
Illegal SapI.rc site found at 1596
Design Notes
Cell culture
293T cells were maintained in Dulbecco’s Modified Eagle Medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS; Gibco, Rockville, MD, USA), 100 mg/mL of streptomycin, and 100 unit/mL of penicillin at 37 °C in 5% CO2. HEK293T cells transfected with human ACE2 (293T-ACE2) were cultured under the same conditions with the addition of G418 (0.5 mg/mL) to the medium.
Source
HEK293T cells and ACE2-293T cells (cells transfected with human ACE2) were purchased from ProCell (Wuhan, China).
Serum sample (harvested at Day 35 post vaccination by an RBD vaccine was used for infectivity inhibition experiment. Detailed information is provided by Yang et al, Nature 2020 and was a kindly gift from Dr. Jingyun Yang (West China Hospital, Sichuan University).