Plasmid_Backbone

Part:BBa_K3682000:Design

Designed by: YOU-CHENG LEE   Group: iGEM20_NYMU-Taipei   (2020-10-15)


Bacterial expression vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1692
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1692
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1692
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

We use PCR to clone this backbone from its original plasmid, and the annealing parts of the primers are TEV cleavage site(forward) and RBS and its later 7 bp sequence. The suspended part of the primers can be determined by whether your method of assembly is(gibson assembly or linear ligation)

Source

From addgene.org id #145730

References

https://www.addgene.org/145730/