Composite

Part:BBa_K367012:Design

Designed by: David Caballero   Group: iGEM10_UPO-Sevilla   (2010-08-26)

fecI & fecR overlapping coding sequences

fecI and fecR genes of Escherichia coli, encoding an ECF sigma factor and a membrane signal transduction protein respectively, involved in signal transduction of the ferric citrate-dependent Fec system. They have been synthesized in the same Biobrick because of their 4 bp overlap in the E. coli genome, not to reduce their expression. FecR protein interacts with the outer membrane sensor FecA and activates the sigma factor FecI, which acts over PfecA promoter region.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 325


Design Notes

Stop codon substituted by standard biobrick TAATAA double stop. PstI site at 1184 removed: CTGCAG to CTGCAA.


Source

This part has been made by PCR and SDM using E. coli K12 genome as template.

References