Terminator

Part:BBa_K3669007:Design

Designed by: Paul Osuna-Kleist   Group: iGEM20_UC_Davis   (2020-10-27)


TcgrA Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TcgrA is already in p502 so we needed regions of homology and for visual construction of our gene constructs.


Source

Comes from cgrA gene of Aspergillus Fumigatus. Primer pairs used in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462 to determine actual terminator location.

References