Regulatory

Part:BBa_K3669003:Design

Designed by: Paul Osuna-Kleist   Group: iGEM20_UC_Davis   (2020-10-27)


gpdA3B constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

gpdA is not needed to have improved strength in our construct due to the amount of uses. Promoter strength of gpdA3B is greater than that of normal gpdA which only has one cope of the gpdA box, as opposed to three.


Source

Part originally from the minimal gpdA promoter in Aspergillus Nidulans He Zhang, Jian nan Yan, Hui Zhang, Tian qi Liu, Yue Xu, Yuan yuan Zhang, Jie Li, Effect of gpd box copy numbers in the gpdA promoter of Aspergillus nidulans on its transcription efficiency in Aspergillus niger, FEMS Microbiology Letters, Volume 365, Issue 15, August 2018, fny154,

References