Regulatory
Part:BBa_K3661006:Design
Designed by: Weixuan Lu Group: iGEM20_CPU_CHINA (2020-10-23)
JTi2(NF-kb induced promoter)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
we have considered about changing the repeats number in the binding sites, and eventually decided to apply 5 times as the old part did.
Source
Homo genome,sequence found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/NM_001078 Iademarco MF, McQuillan JJ, Rosen GD, Dean DC. Characterization of the promoter for vascular cell adhesion molecule-1 (VCAM-1). J Biol Chem. 1992 Aug 15;267(23):16323-9. PMID: 1379595.