RNA

Part:BBa_K3658034:Design

Designed by: Yujeong Oh   Group: iGEM20_NYU_Abu_Dhabi   (2020-10-27)


Bsal guide RNA for CRISPR Cas12a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first ensured our LAMP/RPA primers fell within the range of our PCR primers. We then selected as our gRNA target an appropriate region that falls within our RPA/LAMP primers in order to create our guide RNAs. When multiple gRNA options were provided, the ones with the highest off-target score were selected. The selected gRNA were then NCBI blasted to make sure that they are specific to the chosen bacterial strain.


Source

We found the Bd ITS region sequence in NCBI and used Benchling to design the gRNA.

References