Coding

Part:BBa_K3657053

Designed by: Elizaveta Chernova   Group: iGEM20_Heidelberg   (2020-10-25)


Lambda N - KRAB fusion protein

This part is a fusion protein consisting of a viral antiterminal protein lambda N (BBa_K3657014) and a KRAB repressor protein (BBa_K2549055).

Lambda N binds to the BoxB RNA hairpin (BBa_K3657015). In this way the KRAB protein can be attracted to a specific RNA, for example an RNA that forms a triple helix with DNA or a guide RNA of a dCas. If the RNA binds in front of a promoter, this construct will result in repression of the gene of interest.

More information available here: iGEM Heidelberg 2020

Sequence and Features
Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

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Categories
Parameters
None