Composite

Part:BBa_K3657044

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-22)


Split-Tetrahymena TransTransSplicing Guide with mScarlet

Single-guide RNA for use with the catalytic core of a Tetrahymena thermophila trans-splicing ribozyme. Splicing results in the replacement of the 3' intron of the target sequence with mScarlet. For more details see the design section of this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 688
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None