Coding

Part:BBa_K3657012:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)


RNA binding Pentatricopeptide Repeat Protein - Motif ACACAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


PPR Proteins binding a specific RNA sequence can be assebled, usin the base-specific domains:

Adenine:BBa_K3657006

Guanine:BBa_K3657007

Uracil:BBa_K3657008

Cytosine:BBa_K3657009

For more information on the design, visit iGEM Heidelberg 2020

Source

Arabidopsis thaliana

References

S. Aubourg and N. Boudet and M. Kreis and A. Lecharny (2004) In Arabidopsis thaliana, 1% of the genome codes for a novel protein family unique to plants.Plant Molecular Biology 42, 603-613

S. Manna (2015) An overview of pentatricopeptide repeat proteins and their applications..Biochimie 113, 93-9

A. Barkan and Margarita Rojas and S. Fujii and Aaron Yap and Y. S. Chong and C. Bond and I. Small (2012) A Combinatorial Amino Acid Code for RNA Recognition by Pentatricopeptide Repeat Proteins.PLoS Genetics 8