Part:BBa_K3657009:Design

RNA binding Pentatricopeptide Repeat Protein (Guanine)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

PPRs are specific for the different RNA bases, by varying the amino acids at position 3 and 34. For the guanine binding PPRs, they are threonine and aspartic acid, respectively. For more information on the design, visit iGEM Heidelberg 2020

Source

Arabidopsis thaliana

References

S. Aubourg and N. Boudet and M. Kreis and A. Lecharny (2004) In Arabidopsis thaliana, 1% of the genome codes for a novel protein family unique to plants.Plant Molecular Biology 42, 603-613

S. Manna (2015) An overview of pentatricopeptide repeat proteins and their applications..Biochimie 113, 93-9

A. Barkan and Margarita Rojas and S. Fujii and Aaron Yap and Y. S. Chong and C. Bond and I. Small (2012) A Combinatorial Amino Acid Code for RNA Recognition by Pentatricopeptide Repeat Proteins.PLoS Genetics 8