Coding

Part:BBa_K3657005:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)


RNA binding Pumby Protein - Motif AUGGUU


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 184
    Illegal XhoI site found at 517
    Illegal XhoI site found at 628
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Pumby Proteins up to 18 subunits can be assebled, usin the base-specific domains:

Adenine:BBa_K3657000

Guanine:BBa_K3657001

Uracil:BBa_K3657002

Cytosine:BBa_K3657003

For more information on the design, visit iGEM Heidelberg 2020

Source

Drosophila Melanogaster (Part of the Puf protein)

References

Shuyun Dong and Yang Wang and C. Cassidy-Amstutz and Gang Lu and R. Bigler and Mark R. Jezyk and C. Li and T. Hall and Z. Wang (2011) Specific and Modular Binding Code for Cytosine Recognition in Pumilio/FBF (PUF) RNA-binding Domains*.The Journal of Biological Chemistry 286, 26732 - 26742

Katarzyna P Adamala and D. Martin-Alarcon and E. Boyden (2016) Programmable RNA-binding protein composed of repeats of a single modular unit.Proceedings of the National Academy of Sciences 113, E2579 - E2588