Coding

Part:BBa_K3656305

Designed by: Huang feiyu   Group: iGEM20_AHUT-ZJU-China   (2020-10-23)


OT3-Carbonic Anhydrase-Wild Type

This part is the coding sequence of the Pyrococcus horikoshii OT3 Carbonic Anhydrase Wild Type(OT3-CA-WT). It is a γ-class carbonic anhydrase，with high thermal stability(50 to over 125℃) but low catalytic activity. In our project, it is the original sequence. Based on this sequence, We have processed and modified it in order to improve its catalytic activity. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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