Part:BBa_K365005:Design
20 aa linker RFC-25 (fusion standard Freiburg)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The linker has been order as six separate, EcoRI+AgeI precut primers, which were hybridized in order to obtain the complete linker sequence.
Forward: 5’aattcgcggccgcttctagatggc
3’
Forward: 5’cggcGCGAGCGGCGCGGGCGGCAGCGAAGGCGGCGGCAG
3’
Forward:
5’CGAAGGCGGCACCAGCGGCGCGACCa 3’
Reverse: 5’ccggtGGTCGCGCCG
3’
Reverse:
5’CTGGTGCCGCCTTCGCTGCCGCCGCCTTCGCTGCC 3’
Reverse:
5’GCCCGCGCCGCTCGCgccggccatctagaagcggccgcg3’
The linker has been order as six separate, EcoRI+AgeI precut primers, which were hybridized in order to obtain the complete linker sequence.
Sauer, R. T., T. A. Baker, et al. (2009). "Structures of asymmetric ClpX hexamers reveal nucleotide-dependent motions in a AAA+ protein-unfolding machine." Cell 139(4): 744-56.
Forward strand (5’->3’) : (89 bp)
aattcgcggccgcttctagatggc|cggcGCGAGCGGCGCGGGCGGCAGCGAAGGCGGCGGCAG|CGAAGGCGGCACCAGCGGCGCGACCa
Reverse strand (5’-3’) : (89 bp)
ccggtGGTCGCGCCG|CTGGTGCCGCCTTCGCTGCCGCCGCCTTCGCTGCC|GCCCGCGCCGCTCGCgccggccatctagaagcggccgcg
Ordered primer:
Source
References