RNA

Part:BBa_K3645016:Design

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-27)


sgRNA scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 135
    Illegal BsaI.rc site found at 37


Design Notes

The scaffold of sgRNA already contains a terminator domain, so to use this part we only need to replace the guide sequence.


Source

From Peking iGEM 2019

References