Composite

Part:BBa_K3645013:Design

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-26)


CBE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2338
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6014
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4617
    Illegal XhoI site found at 5623
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This can express the CBE protein when arabinose exists in the medium. Needs sgRNA to be fully functional


Source

Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A. Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Apr;3(4):423-429. doi: 10.1038/s41564-017-0102-6. Epub 2018 Feb 5. PMID: 29403014.

References