Coding
Part:BBa_K3645005:Design
Designed by: Huang nan Group: iGEM20_Peking (2020-10-26)
UGI (codon optimized)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a frequently used part in CBE designs for higher editing efficiency
Source
Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR. A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul;583(7817):631-637. doi: 10.1038/s41586-020-2477-4. Epub 2020 Jul 8. PMID: 32641830; PMCID: PMC7381381.