Coding

Part:BBa_K3645003:Design

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-26)


G1397 DddA(tox)-N (no start codon)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 45
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that this sequence has no start codon


Source

Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR. A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul;583(7817):631-637. doi: 10.1038/s41586-020-2477-4. Epub 2020 Jul 8. PMID: 32641830; PMCID: PMC7381381.


References