Plasmid

Part:BBa_K3644008:Design

Designed by: Lam Kai Ching   Group: iGEM20_Hong_Kong_HKU   (2020-10-27)


Bacteria codon-optimized RfxCas13d protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 517
    Illegal EcoRI site found at 937
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 517
    Illegal EcoRI site found at 937
    Illegal NotI site found at 2926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 517
    Illegal EcoRI site found at 937
    Illegal BamHI site found at 2917
    Illegal XhoI site found at 2392
    Illegal XhoI site found at 2935
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 517
    Illegal EcoRI site found at 937
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 517
    Illegal EcoRI site found at 937
    Illegal NgoMIV site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein-coding sequence in this plasmid is codon-optimized for bacteria.


Source

This sequence is extracted from a paper.

References