Composite

Part:BBa_K364323:Experience

Designed by: Endre Károly Kristóf   Group: iGEM10_Debrecen-Hungary   (2010-10-20)


Physiological function of NHR-31

The nhr-31 activity is required for proper growth, development, and function of the excretory cell. In regulating excretory cell development, NHR-31 appears to function by controlling the expression of genes encoding subunits of the vacuolar ATPase.

Potential applications of GAL4-NHR-31

By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364323

Picture of gel electrophoresis: Gal4-NHR31 in pCDNA3.1 resulting an insert of 974 bp.
Picture of Western blot: Gal4-NHR31 composite construct is highly expressed after transferring it into pCDNA3.1

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