Coding
Part:BBa_K364321:Design
Designed by: Endre Károly Kristóf Group: iGEM10_Debrecen-Hungary (2010-10-20)
NHR-64 LBD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 178
Design Notes
The reconstruction of the LBD and Backbone will be done with RFC 25.
Source
Arteficially synthetized Ligand Binding domain of C. elegans nuclear receptor NHR-64. This part is placed into pSB1C3 carrier vector.
References
Liang B, Ferguson K, Kadyk L, Watts JL, 2010. The Role of Nuclear Receptor NHR-64 in Fat Storage Regulation in Caenorhabditis elegans. PLoS ONE 5(3): e9869. doi:10.1371/journal.pone.0009869