Composite

Part:BBa_K364320:Experience

Designed by: Endre Károly Kristóf   Group: iGEM10_Debrecen-Hungary   (2010-10-20)


Physiological effects of NHR-8

NHR-8 is required for gut function and may respond to the food signal.

The nhr-8 gene mutants have abnormally low resistance to the toxins colchicine and chloroquine.This resembles the regulation of protective metabolic enzymes by xenobiotic-sensing nuclear receptors.

NHR-8 may thus mediate a subset of the resistance traits promoted by PGP-3.

Possible applications of GAL4-NHR-8

By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364320

Picture of gel electrophoresis: Gal4-NHR8 in pCDNA3.1 resulting an insert of 953 bp.
Picture of Western blot: Gal4-NHR8 composite construct is highly expressed after transferring it into pCDNA3.1
Activation of Gal-NHR8 and Gal-NHR64 to oil sand extractions.
Activation of NHR8 to Blesse bleu cheese extract.

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