Coding

Part:BBa_K364316:Design

Designed by: Lior Malka   Group: iGEM10_Debrecen-Hungary   (2010-10-01)

NHR-25 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of the LBD and pSB1C3 backbone have been done with RFC 25.

Source

C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-25 placed into pSB1C3 carrier vector.

References

1. Brooks DR, Appleford PJ, Murray L, Isaac RE. "An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensin-converting enzyme family that lacks a metallopeptidase active site." [http://www.jbc.org/content/278/52/52340.long2003 J Biol Chem. Dec 26; Epub 2003 Oct 14.]