Part:BBa_K364306

Human CAR LBD

The constitutive androstane receptor (CAR) also known as nuclear receptor subfamily 1, group I, member 3 is a protein that in humans is encoded by the NR1I3 gene.CAR is a member of the nuclear receptor superfamily and along with PXR functions as a sensor of endobiotic and xenobiotic substances and in response upregulates the expression of proteins responsible for the metabolism and excretion of these substances. Hence CAR (and PXR) are important in the detoxification of foreign substances such as drugs.

More on CAR here: [http://en.wikipedia.org/wiki/Constitutive_androstane_receptor].

CAR Uniprot: [http://www.uniprot.org/uniprot/Q14994].

View the murine CAR lingand binding domain crystal structure here: [http://www.pdb.org/pdb/explore/jmol.do?structureId=1XNX&bionumber=1].

Picture of murine CAR ligand binding domain with a specific inverse agonist Androstenol.

Description of the murine CAR LBD crystal structure with reference here: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727924/?tool=pubmed].

Picture of gelelectrophoresis: human CAR ligand binding domain in pSB1C3 restricted with EcoRI PstI.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 113
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]