Coding

Part:BBa_K3631000:Design

Designed by: Sihan Ju   Group: iGEM20_NJMU-China   (2020-10-21)


Part A of Serotonin Whole Cell Biosensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 157
    Illegal PstI site found at 565
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 157
    Illegal PstI site found at 565
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 85
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 157
    Illegal PstI site found at 565
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 157
    Illegal PstI site found at 565
    Illegal NgoMIV site found at 412
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The chosen promoter should facilitate our regulation and its expression in E. coli. 1. Rampioni G, Bertani I, Zennaro E, Polticelli F, Venturi V, Leoni L. The quorum-sensing negative regulator RsaL of Pseudomonas aeruginosa binds to the lasI promoter. J Bacteriol. 2006 Jan;188(2):815-9. doi: 10.1128/JB.188.2.815-819.2006. PMID: 16385073; PMCID: PMC1347304. 2. Knecht LD, O'Connor G, Mittal R, Liu XZ, Daftarian P, Deo SK, Daunert S. Serotonin Activates Bacterial Quorum Sensing and Enhances the Virulence of Pseudomonas aeruginosa in the Host. EBioMedicine. 2016 Jul;9:161-169. doi: 10.1016/j.ebiom.2016.05.037. Epub 2016 May 28. Erratum in: EBioMedicine. 2017 Sep;23 :195. Pasini, Patrizia [removed]. PMID: 27333040; PMCID: PMC4972532.

Source

It is cloned from lasR gene in Pseudomonas aeruginosa. Sequences was acquired from NCBI: https://www.ncbi.nlm.nih.gov/gene/881789.

References