Coding

Part:BBa_K363008

Designed by: Justin Porter   Group: iGEM10_Johns_Hopkins   (2010-10-26)

Cch1, voltage gated calcium channel from S. cerevisiae

This gene codes for the Cch1 protein from Saccharomyces cerevisiae. It codes for a high affinity voltage gated calcium channel. In vivo, this channel responds to mating pheromones, hypertonic stress, various forms of ER stress (such as accumulation of misfolded proteins), amiodarone treatment, high pH, high iron, and externally applied electric fields.

This gene, complete with sequence, is the wild-type version of the parts BBa_K222004, submitted by Team Valencia without a sequence.

Usage and Biology

This wild-type part was used in experiments characterizing in in concert with a transcriptional activator by the Johns Hopkins Team in 2010 here.

This part was also used to characterize the behavior of the fluorescent enzyme aequorin by the Valencia Team in 2009 here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6026


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Categories
Parameters
None