Regulatory

Part:BBa_K363001:Design

Designed by: Noah Young   Group: iGEM10_Johns_Hopkins   (2010-10-25)

The consensus calcium dependent response element binding site for the Crz1 activator.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is NOT itself a promoter. It must be inserted upstream of a promoter to function.


Source

This part was derived from calcium dependent response element (CDRE) binding sequence of the FKS2 gene. The consensus sequence for Crz1 binding as described by Yoshimoto et al (2002) was mutated at non conserved residues to generate this part. This UAS is the consensus sequence for Crz1 binding, and is expected to have higher binding than the UAS characterized in BBa_K363006, as it is the consensus sequence and occurs with ~4 times higher frequency than the characterized sequence.

References

Yoshimoto et al, J Biol Chem. 2002 Aug 23;277(34):31079-88. Epub 2002 Jun 10. Genome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiae.