Coding
Part:BBa_K3629019:Design
Designed by: Arshia Mostoufi Group: iGEM20_Calgary (2020-10-09)
Yarrowia lipolytica LEU4 gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 728
Illegal BamHI site found at 293
Illegal XhoI site found at 428 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 799
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 544
Design Notes
Below is the codon-optimized sequence of Yarrowia lipolytica LEU4 for use in any expression construct. However, we have also made our own expression construct BBa_K3629023, designed to allow for optimal expression of LEU4 in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.
Source
From genome sequence of Yarrowia lipolytica CDS: T01033.