Coding

Part:BBa_K3629008:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-12)


Modified Trichoderma reesei EGI with 6X His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression and function in Yarrowia lipolytica. The native signal peptide from T. reesei was also removed so it would not interfere with fused secretion tags native to Y. lipolytica

Source

From genomic sequence of Trichoderma reesei.

References