Protein_Domain

Part:BBa_K3629003:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-10)


Yarrowia lipolytica TEF1 promoter with first half of Lip2 signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 53
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First half of Lip2 signal peptide codon optimized for expression and function in Yarrowia lipolytica.


Source

From the genomic sequence of wild-type Y. lipolytica strain CLIB122.

References