Coding

Part:BBa_K3621002:Design

Designed by: Sangita Vasikaran   Group: iGEM20_MIT   (2020-10-04)


Cas6-> cleaves cut site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
    Illegal NotI site found at 71
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
    Illegal NgoMIV site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was planned and designed online for use in our computational model. See more on the Model Page on the MIT iGEM 2020 wiki.

Source

This part was from iGEM and the Weiss lab.

References