Coding

Part:BBa_K3619012:Design

Designed by: Isak Ingerholt   Group: iGEM20_Lund   (2020-09-13)


cDNA encoding the antimicrobial peptide Temporin-1Ta


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence in codon optimized for expression in E.coli K12.


Source

Temporin-1Ta originates from Rene temporia. DNA sequence was obtained through back translation of peptide found in the Uniprot record P56917.

References

1. https://www.uniprot.org/uniprot/P56917

2. https://webs.iiitd.edu.in/raghava/cancerppd/refpdf/9022710.pdf

3. https://pubmed.ncbi.nlm.nih.gov/10691983/