Coding

Part:BBa_K3619011:Design

Designed by: Isak Ingerholt   Group: iGEM20_Lund   (2020-09-13)


Gene scar, encoding the antimicrobial peptide Scarabaecin.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon optimized for expression in E.coli K12.


Source

The scar gene originates from Oryctes rhinoceros. DNA sequence was obtained from back translation of the peptide scarabaecin.

References

1. https://www.uniprot.org/uniprot/Q86SC0

2. https://www.sciencedirect.com/science/article/abs/pii/S0006291X03011628

3. https://pubmed.ncbi.nlm.nih.gov/12676931/