Coding

Part:BBa_K3617008:Design

Designed by: David Nørgaard Essenbæk   Group: iGEM20_UCopenhagen   (2020-10-21)


GPA1 with TEV protease cleavage sequence 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cleavage sites where chosen by using an alignment of bovine G-α and GPA1 and inspecting the crystal structure of bovine G-α and at the same time taking into consideration residues and regions that have been investigated in mutagenesis studies.


Source

GPA1 from S. cerevisiae.

References